Extended Data Fig. 1: Multiple isotypes of the 3BNC117 antibody are expressed by engineered B cells.
From: In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice
a–d. ELISA for each isotype. a. IgM, b. IgG1, c. IgG2c and d. IgA. All samples come from the CMV-Cas9gRNA + donor injected mice at different time points, as indicated in each legend. Mean and SD are indicated. n = 3 biologically independent animals. e,f. Area under the curve (AUC) for A-D. e. IgM, ns; pv = 0.1288, ***; pv = 0.0008, **; pv = 0.0062, f. IgG1, ns; pv = 0.131 and from top to bottom as presented in the graph, **; pv = 0.0044 and pv = 0.0013, g. IgG2c, ***; pv = 0.0007, *; pv = 0.0195, **; pv = 0.0098, h. IgA, ns; pv = 0.0587, **; = pv = 0.0013, *; pv = 0.0403, for two-sided unpaired t-test. n = 3 biologically independent animals. For A-H, sample collection day is indicated. Mean values are indicated. i. Fraction of 3BNC117 IgG titers as quantified by ELISA using purified gp120 binding sera from donor injected mice immunized with gp120, at day 37. ***; pv = 0.0007 for two-sided unpaired t-test. n = 3 biologically independent samples. Mean values are indicated.
