Fig. 3: TACT assays are concordant with gold standard ELLA and ELISpot assays.
From: Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR
a–d, Concordance between assays to quantify cellular immunity. The quantification shown is with the DMSO control subtracted from the spike peptide-stimulated sample. Each dot represents a unique participant color coded based on their COVID-19 and vaccination statuses (legend). The dashed line represents thresholds for each assay. a, Concordance between ELLA and qTACT assays. For 117 participants, IFN-γ protein secretion was quantified by ELLA (y axis) and CXCL10 mRNA by qTACT (x axis). b, Concordance between ELLA and dqTACT assays. For 133 participants, IFN-γ protein secretion was quantified by ELLA (y axis) and CXCL10 mRNA by dqTACT (x axis). c, Concordance between ELISpot and qTACT assays. For 50 participants, IFN-γ producing cells were quantified by ELISpot (y axis) and CXCL10 mRNA by qTACT (x axis). d, Concordance between ELISpot and dqTACT assays. For 46 participants, IFN-γ producing cells were quantified by ELISpot (y axis) and CXCL10 mRNA by dqTACT (x axis).
