Extended Data Fig. 3: Comparing TACTseq assay to other T cell assays and dqTACT on different PCR machines.

A. Concordance between ELLA and TACTseq assays. For 24 subjects, IFN-γ secretion was quantified by ELLA (y-axis) and CXCL10 mRNA by TACTseq (x-axis). The quantification shown is with the DMSO control subtracted from the spike peptide stimulated sample. Each dot represents a unique subject color coded based on their COVID-19 and vaccination statuses. The dashed line represents thresholds for each assay. B. Concordance between ELISpot and TACTseq assays. For 24 subjects, IFN-γ producing cells were quantified by ELISpot (y-axis) and CXCL10 mRNA by TACTseq (x-axis). The quantification shown is with the DMSO control subtracted from the spike peptide stimulated sample. Each dot represents a unique subject color coded based on their COVID-19 and vaccination statuses. The dashed line represents thresholds for each assay. C. Concordance between ELISpot and ELLA assays. For 85 samples, IFN-γ protein secretion was quantified by ELLA (y-axis) and IFN-γ producing cells were quantified by ELISpot (y-axis). The quantification shown is with the DMSO control subtracted from the spike peptide stimulated sample. Each dot represents a unique subject color coded based on their COVID-19 and vaccination statuses. The dashed line represents thresholds for each assay. D. Nonparametric Spearman’s correlation between dqTACT samples run on Bio-Rad CFX384 (y-axis) and Hyris bCUBE 2.0 (x-axis). Bands represent 95% confidence interval. E. Nonparametric Spearman’s correlation between dqTACT samples run on Bio-Rad CFX96 (y-axis) and Applied Biosystems AB 7500 (x-axis). Bands represent 95% confidence interval.