Extended Data Fig. 10: In vivo gene activation by Red-CPTS upon noninvasive red light illumination.
From: A red light–responsive photoswitch for deep tissue optogenetics
(a, b) ICR mice were transfected with plasmids encoding Red-CPTS and luciferase reporter together with a plasmid encoding unrelated sgRNA as a negative control (a, Empty) or sgRNA targeting GAL4UAS (b, GAL4UAS). After the transfection, the mice were noninvasively illuminated at 660 nm or kept in the dark as shown in Supplementary Figure 28, and then bioluminescence imaging of the mice was performed. (c) Total bioluminescence intensities of the mice shown in a and b. Gray and red bars represent the mean ± s.d., and dots represent the total bioluminescence intensity of each mouse (n = 4 mice per group). (N.S. P > 0.05; ****P < 0.0001; using two-way ANOVA with multiple comparisons). (d) Red light-dependent endogenous gene activation by Red-CPTS with unrelated sgRNA as a negative control (Empty) or sgRNA targeting mouse ASCL1 (mASCL1) in vivo in living BALB/c mice. Data are represented as the relative mRNA level to the non-transfected negative control (n = 6 mice per group). Gray and red bars represent the mean ± s.d., and dots represent individual data points. No difference can be observed in the appearance between the mice maintained in the dark and the ones illuminated with red light at 660 nm for 16 h. P values are indicated above the bars. (N.S., not significant P > 0.05; ****P < 0.0001; using two-way ANOVA with multiple comparisons).
