Extended Data Fig. 2: Red-CPTS can be actively switched off using 800-nm light illumination.
From: A red light–responsive photoswitch for deep tissue optogenetics
(a-d) After the pre-activation of Red-CPTS by the 660-nm illumination for 12 h, the cells were further incubated under the 660-nm illumination condition (a) or switched to the dark condition (b) or the 800-nm illumination condition (c). As a control, the sample was incubated under the dark condition throughout the experiment (d). ASCL1 mRNA levels were measured at 0 h, 0.75 h, 1.5 h and 3.0 h as shown in the figure. Box plots show the median (center line), first and third quartiles (box edges), 1× the SD (whiskers), and individual data points. (n = 6 biologically independent samples, mean ± s.d.).
