Extended Data Fig. 3: Comparison of Red-CPTS and RpBphP1-PspR2/QPAS1-based CPTS at different illumination intensities.
From: A red light–responsive photoswitch for deep tissue optogenetics
(a-c) Fluc reporter gene activation by Red-CPTS (a), RpBphP1-PpsR2-based CPTS (b), and RpBphP1-QPAS1-based CPTS (c) at various illumination intensities. RpBphP1-PpsR2/QPAS1-based CPTS has configuration #3 shown in Fig. 3b, c. Experimental conditions are same as those in Fig. 3c, d except for the illumination intensities. Ratios of the mean bioluminescence intensity under the red light condition (red bar) to that under the dark condition (gray bar) are depicted above the bars. Bar data are shown as the mean ± s.d. from four biological replicates. Dots represent individual data points. (d) Left: Comparison of the Light/Dark contrasts between Red-CPTS and RpBphP1-PpsR2/QPAS1-based CPTS at different illumination intensities. Right: The cropped data with the mean Light/Dark contrast of 0 to 3.
