Fig. 4: Clinical parameters of SARS-COV-2-infected and treated Roborovski dwarf hamsters.

a, Design of the Roborovski dwarf hamster study. Animals were infected on day 0 with 10⁵ PFU of SARS-CoV-2 Alpha (B.1.1.7) variant. Treatment was administered either directly after infection (0 hours p.i.) or 1 day after infection (24 hours p.i). For each treatment group, 12 animals were injected i.p. with 10 mg kg⁻¹ of ensovibep, 10 mg kg⁻¹ of mAb cocktail (5 m kg⁻¹ of REGN10933 and 5 mg kg⁻¹ of REGN10987) or PBS (placebo). Additionally, a group of six non-infected and non-treated control animals was included as comparators for the infected and treated groups. Daily measurement of body weights and temperatures as well as observation of clinical symptoms was undertaken. Animals were sacrificed on day 3 or day 5 p.i. or immediately once an individual animal reached a defined humane endpoint. b, Survival of animals for 5 days p.i. Animals that had to be euthanized according to defined humane endpoints were considered as non-survived. Body weights (c) and body temperatures (d) throughout the study duration. Data points show mean ± s.d. of the following number of animals analyzed per treatment group at 0/1/2/3/4/5 days p.i.: ensovibep 0 hours: n = 10/10/10/10/5/5; mAb cocktail 0 hours: n = 9/9/9/9/5/5; ensovibep 24 hours: n = 10/10/10/10/5/5; mAb cocktail 24 hours: n = 12/12/12/7/6/6; placebo, infected: n = 12/12/12/7/4/4; placebo, non-infected: n = 6/6/6/6/3/3. Some animals were excluded from the final analysis due to low drug exposure, likely due to a failure of i.p. injections. These animals were excluded from all analyses. Lines connecting dots are interrupted for any change in animal numbers between consecutive days. Because a considerable number of animals in the mAb cocktail and placebo groups reached defined humane endpoints by day 2 p.i., this day is zoomed-in. Red symbols: animals taken out of the study at day 2 due to severe clinical symptoms. Data are represented by the median and values for individual animals. Number of biologically independent animals: ensovibep 0 hours: n = 10; mAb cocktail 0 hours: n = 9; ensovibep 24 hours: n = 10; mAb cocktail 24 hours: n = 12; placebo, infected: n = 12; placebo, non-infected: n = 6. NS, not significant.