Extended Data Fig. 7: Evaluation of small molecule inhibitor cocktails in primary human T cells. | Nature Biotechnology

Extended Data Fig. 7: Evaluation of small molecule inhibitor cocktails in primary human T cells.

From: High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails

Extended Data Fig. 7: Evaluation of small molecule inhibitor cocktails in primary human T cells.

(a) Evaluation of relative increase in percent knock-in using an ssDNA CD5-HA knock-in construct over varied concentrations of 5 different small molecule inhibitors assessed by flow cytometry. Red bars indicate concentrations chosen for subsequent experiments. (b) Comparison of relative percent knock-in (top), live cell counts (middle), and viability with Ghost Dye 780 (Tonbo) (bottom) with small molecule inhibitor combinations. Cocktails chosen for subsequent experiments are highlighted in red (M3814), blue (MT) and yellow (MTX). (c-d) Evaluation of Novobiocin effects on (c) live cell counts and (d) knock-in efficiency using a small CD5-HA ssDNA HDRT. (e) Evaluation of Novobiocin effects on knockin efficiency at varied concentration using a small CD5-HA ssDNA HDRT in combination with M3814, MT, and MTX inhibitors. Each experiment was performed with T cells from 2 independent healthy human blood donors represented by individual dots + mean. M = M3814, MT = M3814 + Trichostatin A, MTX = M3814 + Trichostatin A + XL413, NVB = Novobiocin.

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