Extended Data Fig. 1: Illustration of ssCTS designs. | Nature Biotechnology

Extended Data Fig. 1: Illustration of ssCTS designs.

From: High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails

Extended Data Fig. 1: Illustration of ssCTS designs.

Detailed illustrations of CTS designs evaluated in the manuscript highlighting the location and orientation of gRNA target sequences (red), 4 bp mismatch (pink), PAM interaction site (yellow), and transgene (blue). (a-j) Illustration of short CD5-HA HDRT designs evaluated in Fig. 1a–c and Extended Data Fig. 2c. (k) Representative illustration of optimal ssCTS design used for large HDRTs throughout the manuscript. CTS = Cas9 Target Site, ssCTS = ssDNA HDRT + CTS sites, HDRT = homology-directed-repair template.

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