Extended Data Fig. 4: UB organoid differentiation protocol is efficient when using hiPSCs. | Nature Biotechnology

Extended Data Fig. 4: UB organoid differentiation protocol is efficient when using hiPSCs.

From: Human ureteric bud organoids recapitulate branching morphogenesis and differentiate into functional collecting duct cell types

Extended Data Fig. 4: UB organoid differentiation protocol is efficient when using hiPSCs.The alternative text for this image may have been generated using AI.

a, Using the hiPSC line BJFF.6, a 30 hour exposure during the first stage of differentiation was also required for the primitive streak phenotype. b, AIM transcription factors PAX2, GATA3, LHX1, and HOXB7 were induced with very high efficiency by day 3. c, The hiPSC-derived AIM exhibited efficient formation of nephric duct spheroids in 96-well low attachment plates. The spheroids then underwent similar molecular and morphological development compared to those derived from hESCs as shown in Fig. 1. d, Nephric duct spheroids efficiently grew into branched UB organoids. e-f, hiPSC-derived UB organoids exhibited tip-stalk patterning and spontaneously formed differentiated AQP2-positive principal cells. Scale bars, 50 μm (a-d) and 100 μm (e-f).

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