Extended Data Fig. 8: Analysis of the effect of flanking mutations on cellular fitness.

a, Log₂ values for read count ratios (day 8/day 2) for cell lineages edited with repair templates harboring the original E-box sequence and a unique sequence tag. b, Log₂ values for read count ratios (day 8/day 2) for cell lineage pairs with a unique sequence tag after calculating the ratio of read counts for mutated vs. original sequence at each time point. In panels a and b, the results are shown for two independent replicate experiments, showing the sequence tags that were recovered above cut-off (read count >50 on day 2) from both replicates. For PAICS, SHMT2, and PPAT, sequence tags harboring two flanking mutations are shown. For RPL23 and HK2, the recovered sequence tags with two mutations were largely different in the two replicate experiments and thus the log₂ values for cell lineages harboring one flanking mutations are shown for them.