Extended Data Fig. 3: The effects of mutating phosphorylation sites in the GRB2 gene on cellular fitness of HAP1 and KBM-7 cells.

Precision editing results are shown for all cell lineages harboring sequence tags with read count >20 on day 2. Log₂ values for read count ratios (day 8/day 2) are shown for each sequence tag pair after calculating the ratio of read counts for mutated vs. original sequence at each time point. Black dots represent independent cell lineages (internal replicates); red lines mark the median values, and p-values from two-sided Wilcoxon signed rank test are shown for each experiment with no multiple comparison adjustments (see Supplementary Table 3 for details of statistical parameters).