Fig. 1: In situ polyadenylation enables spatial profiling of noncoding and nonhost RNAs.

a, Workflow for STRS. b, Comparison of select RNA biotypes between Visium and STRS datasets. The y axis shows the percent of UMIs for each spot. The box shows median and quartile values, and whiskers show 1.5 times interquartile range. c, Detection of coding and noncoding RNAs between Visium and STRS workflows. Color scale shows average log-normalized UMI counts. Dot size shows the percentage of spots in which each RNA was detected. d, Log₁₀-transformed coverage of deduplicated reads mapping to sense (light gray) and antisense (dark gray) strands at the Vaultrc5, ENSMUSG00002075551 and Rps8 loci. Annotations shown are from GENCODE M28 and include one of the five isoforms for Rps8 as well as the four intragenic features within introns of Rps8. e, Spatial maps of coding and noncoding transcripts for Visium and STRS workflows. Spots in which the transcript was not detected are shown as gray. Color scale indicates log-normalized expression. f, Detection of REOV transcripts using the standard workflow, STRS and STRS with targeted pulldown enrichment. Spots in which the virus was not detected are shown as gray.