Fig. 5: Characterization of TadCBEs using a genomically integrated mESC target sequence library.
a, Overall efficiency and selectivity of base editors analyzed through editing of the library. Data show the average fraction of edited sequencing reads across all library members between protospacer positions −9 to 20, where positions 21–23 are the PAM. b, BE4max, TadCBEa–e, TadCBEd V106W and TadDE editing profiles across 10,638 genomically integrated target sites. The editing window is defined as the protospacer positions for which average editing efficiency is ≥30% of the average peak editing efficiency. Window plots for all variants tested in the library experiment can be found in Supplementary Fig. 28. c, Sequence motifs of TadCBEd and TadCBEd V106W for cytosine and adenine base editing outcomes determined by performing regression on editing efficiencies. Opacity of sequence motifs is proportional to the test R on a held-out set of sequences. Complete sequence motif plots for all variants are in Supplementary Fig. 30.
