Extended Data Fig. 6: Performance comparison of SPOTS to SM-Omics.
From: Integration of whole transcriptome spatial profiling with protein markers
(a) Total mRNA and ADT counts across spatial barcodes recovered in SM-Omics and SPOTS in biological replicates of spleens. (b) Total ADT counts of F4/80 (macrophages) and IgD (B cells) across biological replicates of spleens. (c) Correlations between IgD and F4/80 (normalized ADT counts) for each spatial barcode in spleen by SPOTS and SM-Omics. Note the anti-correlation between F4/80+ macrophage-enriched (Red pulp) and IgD+ B cell-enriched (B follicle) regions in the SPOTS analyses. (d) Differentially expressed ADT levels (Z-score) for each cluster of spatial barcodes in spleen. Left panel: SPOTS, right panel: SM-Omics (e) Heatmap showing differentially expressed mRNAs (Z-score) in each cluster of spatial barcodes in splenic tissues. Left panel: SPOTS, right panel: SM-Omics (f) Visualization of normalized ADT expression of macrophages (F4/80) and B-cells (IgD) in spleens using SPOTS (left) and SM-Omics (right).
