Extended Data Fig. 5: Assessment of misselection between tightly coupled sister chromatids.
From: A spatial genome aligner for resolving chromatin architectures from multiplexed DNA FISH
(a) Schematic of misselection assessment. For every tightly paired sister chromatid, a sliding window of three downstream loci are allowed to exchange with loci on the other sister. Bond probabilities between three upstream loci and three downstream loci are calculated. Original bond probabilities are compared to the cross-over probabilities to evaluate misselection. (b) Bar-plot of misselection frequency per locus on mESC chr 1. (c) Representative image of loci belonging to sister chromatids whose bond probabilities are more probable (-log(Ppredicted/Palternate) > 0) if loci are exchanged between sisters. (d) Number of cross-over events, defined as sequences of misselections of any length, per sister chromatid. (e) Number of misselected loci per sister chromatid. (f) Histogram of length of consecutive misselections across tightly paired sister chromatids. (g). Histogram of length of consecutive correct selections across tightly paired sister chromatids.
