Extended Data Fig. 7: xAM ultrasound imaging of mouse tumors colonized by EcN. | Nature Biotechnology

Extended Data Fig. 7: xAM ultrasound imaging of mouse tumors colonized by EcN.

From: Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria

Extended Data Fig. 7: xAM ultrasound imaging of mouse tumors colonized by EcN.

(a-c) Representative B-mode (top, grayscale) and xAM (bottom, hot-scale) ultrasound images of tumors colonized by pBAD-bARGSer-AxeTxe EcN 1 day after induction with L-arabinose on day 18 (a), 1 day after collapse and re-induction (day 19) (b), or uninduced on day 18 (c). (d) Representative ultrasound images of tumors colonized by pBAD-FP-AxeTxe EcN 1 day after induction with L-arabinose on day 18. Scale bars in (a-d) represent 2 mm. (e) Quantification of the xAM SBR for the same conditions in (a-d) (p values from left to right: 1.0545E-05, 3.73902E-05, 0.487197948). Each group is n = 5 mice and lines indicate the mean. Asterisks represent statistical significance by two-tailed, unpaired Student’s t-tests (*** = p < 0.001, ns = no significance). See Fig. 3a for the corresponding in vivo protocol.

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