Extended Data Fig. 3: Testing bARGSer expression in EcN with IPTG- and aTc-inducible gene circuits. | Nature Biotechnology

Extended Data Fig. 3: Testing bARGSer expression in EcN with IPTG- and aTc-inducible gene circuits.

From: Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria

Extended Data Fig. 3: Testing bARGSer expression in EcN with IPTG- and aTc-inducible gene circuits.

(a) Diagram of the IPTG-inducible construct used to express bARGSer in EcN (top), and representative optical and xAM images of bARGSer-expressing or fluorescent protein (FP)-expressing patches of EcN on solid media with varying IPTG concentrations at 37 °C (bottom). (b) Quantification of the xAM SBR of all patches from the experiment in (a) (n = 8 biologically independent samples examined in one experiment; curves represent the mean). (c-d) Same as in (a-b) but for the aTc-inducible construct. The scale bars in (a,c) represent 1 cm.

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