Fig. 5: High-throughput ISGS identifies cis-regulatory elements determining chromatin organization. | Nature Biotechnology

Fig. 5: High-throughput ISGS identifies cis-regulatory elements determining chromatin organization.

From: Cell-type-specific prediction of 3D chromatin organization enables high-throughput in silico genetic screening

Fig. 5

a, Schematic of impact score calculation from in silico deletion of cis-regulatory elements. b, Schematic of ISGS framework for identifying impactful cis-regulatory elements. c, Visualization of the three attribution methods: GRAM, attention score and impact score. d, Distribution of normalized impact scores in in silico deletion screening. e, Heatmap of ISGS-identified impactful cis-elements. Each row shows a 5-kb locus centered by an impactful 1-kb cis-element, ranked in descent by their impact scores. f, Relative enrichment (Z-score normalized) of ATAC–seq signal and multiple ChIP–seq signals at the four groups of impactful elements. According to the impact score values, cis-elements of each group were further grouped into high-, medium- and low-impact quantile groups when plotting the ChIP–seq/ATAC–seq signals. g, Characterization of in silico screening-identified cis-elements by their genomic annotations.

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