Extended Data Fig. 1: Anti-MuSK target cell characterization. | Nature Biotechnology

Extended Data Fig. 1: Anti-MuSK target cell characterization.

From: Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells

Extended Data Fig. 1: Anti-MuSK target cell characterization.The alternative text for this image may have been generated using AI.

a) Schematic diagram of individual MuSK domain CAARs with a cytoplasmic linker to green fluorescent protein (GFP), generated for epitope mapping. FL, full-length; SP, signal peptide; TMD, transmembrane domain; BBζ, CD137(4-1BB)-CD3ζ. b) Summary of recombinant anti-MuSK B cell receptor (BCR) or mAb specificities. c) MuSK domain CAAR+ Jurkat cells were stained with anti-MuSK mAbs to confirm the domain mapping. d, e) BCRs in primary human IgG+ B cells and Nalm-6 cells expressing each MuSK domain-specific BCR were stained with PE mouse anti-human IgG. f) BCR density was calculated by dividing the number of PE molecules/cell by the surface area (μm2). Mean ± standard deviation of IgG+ B-cells from three individual experiments is shown.

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