Extended Data Fig. 4: Annotating mouse olfactory bulb Slide-seq data31 using an scRNA-seq data32 with TACCO. | Nature Biotechnology

Extended Data Fig. 4: Annotating mouse olfactory bulb Slide-seq data31 using an scRNA-seq data32 with TACCO.

From: TACCO unifies annotation transfer and decomposition of cell identities for single-cell and spatial omics

Extended Data Fig. 4: Annotating mouse olfactory bulb Slide-seq data31 using an scRNA-seq data32 with TACCO.

(a) Compositional cell type annotation. Annotations (color legend) from a single TACCO run on 40 Slide-seq pucks across 2 replicates (replicate 1 in 1st and 3rd columns, replicate 2 in 2nd and 4th columns); (b) Comparison of different annotation strategies. Compositional cell type annotation (first row, color legend) and normalized cell type weights (remaining rows, color bar) from either direct compositional annotation of the spatial data (left column; default TACCO workflow, as in (a)); compositional single cell annotation of the spatial data, and deriving the cell type annotation from the single cell cell-type annotation (middle column); or positional annotation of the single cells in the reference with plotting the categorical cell type annotation at the positions of the beads with the maximum weight per cell (right column).

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