Extended Data Fig. 4: Characterization of targeted dual-mode fluorescent EMcapsulin variants.
From: Genetically encoded barcodes for correlative volume electron microscopy
a, Coomassie-stained Clear-Native PAGE loaded with lysates of HEK293T cells expressing dual-mode fluorescent EMcapsulin variants eUnaG-1M-QtFLAG, eUnaG-2M-QtFLAG or 1M-QteUnaG. b, Unstained UV-illuminated CN-PAGE shown in a. c, Silver-stained SDS PAGE after anti-FLAG pull-down from lysates of HEK293T co-expressing the combinations A, B, and C as specified in the figure. The relative DNA amounts of 1M-QtFLAG or Strep to 1M-Qtanti-mCherry were kept constant at 4:1. d, Densitometric analysis of the conditions A and C shown in c, giving the relative amounts of 1M-QtFLAG with respect to 1M-Qtanti-mCherry (set to 1) obtained from 3 biological replicates (with three technical replicates, mean±SD).
