Extended Data Fig. 10: Minor sequence variations in and near the consensus motif of YY1 fine-tune apparent binding affinities. | Nature Biotechnology

Extended Data Fig. 10: Minor sequence variations in and near the consensus motif of YY1 fine-tune apparent binding affinities.

From: Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq

Extended Data Fig. 10: Minor sequence variations in and near the consensus motif of YY1 fine-tune apparent binding affinities.The alt text for this image may have been generated using AI.

(a) Spike-in normalized sequencing reads per allele and titration point of FLAG-YY1 in F121 mESCs relative to the highest signal at the Qars promoter (Pink: Castaneus, green: 129/Sv). Vertical lines indicating the \(K_d^{Apps}\). Pearson correlation coefficients and two-tailed p-values comparing the fitted and observed relative signal are reported. (b) Binding ratios (log2 scale) of proteins identified by DNA-pulldown followed by mass spec with oligonucleotides identical to the sequences depicted in (a). Blue dot and arrow indicate Yy1.

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