Extended Data Fig. 2: Overview of results of additional BANC-seq experiments. | Nature Biotechnology

Extended Data Fig. 2: Overview of results of additional BANC-seq experiments.

From: Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq

Extended Data Fig. 2: Overview of results of additional BANC-seq experiments.

(a) Venn diagrams of overlap between sites with fitted high affinity \(K_d^{Apps}\) by BANC-seq and endogenous ChIP-seq peaks of the respective transcription factor in the respective organism. (b) Distribution of \(K_d^{Apps}\) of MYC and YY1 in MCF-7 nuclei. BANC-seq experiments were performed using 5 titration points, and YY1 apparent binding affinities were probed either with the ChIP-seq or CUT&RUN-based protocol. Dotted lines indicate the tested concentrations per experiment. (c) Heatmap representing spike-in normalized sequencing reads relative to the highest signal for the same experiments as in (b). Each row represents one transcription factor binding site. The overlap of each binding site with peaks from endogenous ChIP-seq experiments of the same transcription factor is shown to the left of each heatmap, while \(K_d^{Apps}\) to the right. (d) Distance (bp) of identified transcription factor binding sites relative to the nearest transcription start site (TSS).

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