Extended Data Fig. 10: Ψ55-dependent basecalling error is restricted to position 55 independent of m1A58 presence.
From: Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing
Heatmap of summed basecalling error frequency of Pus4 KO biological replicate 1 (as in Fig. 4e) categorized by tRNA isoacceptors without an annotated m1A58 (upper panel) or those with an annotated m1A58 (lower panel). The positions of specific RNA modifications in each tRNA are listed in Supplementary Table 18. Nucleotides with higher summed basecalling error frequency relative to WT are in red tones, and those with a lower basecalling error frequency are in blue tones. Ψ55 is indicated by a green arrowhead, m5U54 by a pink arrowhead, and m1A58 by an orange arrowhead.
