Extended Data Fig. 2: Increased ligation time and addition of PEG8000 improves 5′ RNA adapter ligation efficiency.
From: Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing
(a) TBE-Urea gels showing the effect of reaction duration and the addition of 20% PEG8000 on ligation efficiency using commercial S. cerevisiae tRNAPhe as the ligation template. (b) Barplot of the ligation product (tRNAPhe ligated to the 5′ RNA adapter) normalized to an unligated tRNAPhe control. Error bars represent mean ± stdev for n = 3 replicates per condition. P values were determined using a two-sided t-test, *P < 0.05, 2 h 25 °C vs 2 h 25 °C p-value = 0.0241, 20 min 25 °C vs 20 min 25 °C p-value = 0.0450. ON = overnight, PEG = PEG8000 (final concentration of 30%).
