Extended Data Fig. 4: Genomic characterization, expression, conservation, and secondary structure of bktRNA1.
From: RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators
a, Genome-browser plot of RIP-PEN-seq (coverage, blue; 5’-start, red; 3’-end, yellow) for bktRNA1, as well as the evolutionary conservation across 100 vertebrates (green). b, Secondary structure of bktRNA1 in the human genome was predicted by R-scape software. The SHAPE reactivities for each nucleotide were mapped to secondary structures using R2R software. The box H/ACA domain is indicated with a black dashed box, and the backward K-turn structure and the potential K-turn-like structure are marked with green dashed boxes. The NC stem and C stem are indicated with black lines. The blue boxes show the representative motifs. CAB, Cajal body box. c, The SHAPE reactivity signal on bktRNA1. The representative motifs are underlined in the bar plot. The SHAPE reactivity signal was determined by RIP-PEN-SHAPE-MaP in this study.
