Extended Data Fig. 1: Model of an avidite.
From: Sequencing by avidity enables high accuracy with low reagent consumption
(a) side and top views of a modeled avidite. The protein core consists of fluorophore labeled streptavidin. The monomers of tetrameric streptavidin are colored red, blue, green, and yellow. Dye conjugation sites through lysine-NHS chemistry are denoted in the surface rendering as magenta. Fluorophores are not pictured. Avidite arms are associated via a biotin interaction with the core streptavidin protein. Arms are mixed stoichiometrically to achieve averages of three nucleotide containing arms and one linker to additional cores. Molecules conjugated to have been shortened in this representation. (b) Structure of an avidite arm. (c) Structure of the 4-arm linker connecting avidite cores.
