Extended Data Fig. 2: Variance analysis for ncRNA expression levels. | Nature Biotechnology

Extended Data Fig. 2: Variance analysis for ncRNA expression levels.

From: Characterizing expression changes in noncoding RNAs during aging and heterochronic parabiosis across mouse tissues

Extended Data Fig. 2

(A) Principal variance component analysis of biological components (sex, age, tissue, mouse) and technical components (barcode, plate); factors connected with ':' were linearly combined. (B) Visualization of all samples of the TMS cohort over all abundant non-coding RNAs as a t-SNE, colored by sex. (C) Histogram of mean variance calculated in abundant count percentages of all RNA classes. Calculations for each tissue after local filtering over the time course. Threshold determined at 4.5% to separate highly variable and stable tissues.

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