Extended Data Fig. 5: Predicting GSH regions and analyzing Agrobacterium insertion events.
From: Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
a, Schematic overview on annotating rice genomes and predicting conserved GSH regions across 33 rice varieties. b, Schematic overview on using whole genome sequencing to identify insertion events. c, PCR validation of Agrobacteria insertion events as identified by whole-genome sequencing. RB and LB junctions are amplified using primers designed based on surrounding contig sequences. am1 to am10 represent 10 different Agrobacteria-derived mutants. wt represent wild-type rice plants amplified using the junction-spanning primer pairs. d, Overview of Agrobacteria-mediated insertion events and their genome insertion locations.
