Extended Data Fig. 4: Analysis of CCC between CD40LG-transfected NK cells and CD40-transfected B cells with Connectome and NicheNet.
From: Comparative analysis of cell–cell communication at single-cell resolution
a) Circos plot summarizing Connectome’s38 results of significantly differentially-expressed ligand-receptor pair edges between the CD40LG-CD40 transfected condition (shades of red) and GFP-GFP transfected condition (shades of blue). CCC is analyzed between ligands expressed by sender NK cells (bottom) and receptors expressed by receiver B cells (top). b) Dot plot depicting percentage and average expression of differentially-expressed receptors by B cells (top) and ligands by NK cells (bottom) returned by Connectome’s DifferentialConnectome workflow. c) NicheNet20 was applied to predict ligand activities in B cells between the CD40LG-CD40 transfected condition and the GFP-GFP transfected condition. The bar plot depicts pearson coefficient outputs of NicheNet for this analysis. d) Dot plot depicting percentage and average expression of potentially-active ligands shown in (c) by NK cells.
