Extended Data Fig. 3: Flow cytometry and transcriptional analysis of B and NK cell transfection and co-culture.
From: Comparative analysis of cell–cell communication at single-cell resolution
a) Flow cytometry gating scheme used to identify B and NK cells. b) Scatter plots of flow cytometry data showing expression of CD40 and CD40L by B cells and NK cells. Left: B cells and NK cells transfected with GFP-encoding mRNA at start of co-culture. Middle: B cells transfected with CD40-encoding mRNA and NK cells transfected with CD40L-encoding mRNA at start of co-culture. Right: B cells transfected with CD40-encoding mRNA and NK cells transfected with CD40L-encoding mRNA at end of co-culture. For (a-b), percentages of the parent gate are shown for each gate. c) UMAP projections of full dataset colored by cell condition of origin (left) or annotated cell type (right). d) Dot plot depicting average and percent expression of exogenous mRNAs in the four co-cultures.
