Fig. 3: Screening nickases without recognition sequence restriction for mitoBEs.
From: Strand-selective base editing of human mitochondrial DNA using mitoBEs
a, Nickases without sequence restriction or with extensive recognition sequences were screened for mitochondrial base editing at three sites. The mean values from n = 3 biologically independent replicates are shown. b, The editing efficiencies of different mitochondrial sites when left TALE–Nt.BspD6I(C) was combined with right TALE–TadA8e-V106W. c, The editing efficiencies of different mitochondrial sites when right TALE–Nt.BspD6I(C) combined with left TALE–TadA8e-V106W. For b and c, data are presented as mean values ±s.d. of n = 3 independent biological replicates. In a–c, the blue rounded rectangle represents TadA8e-V106W, the red rounded rectangle represents Nt.BspD6I(C) and the gray rectangle represents TALE.
