Extended Data Fig. 2: StcE cleaves mucins in mouse tissues at a maximum tolerated dose of 0.25 mg/kg and exhibits systemic toxicity at higher doses.
From: Design of a mucin-selective protease for targeted degradation of cancer-associated mucins
a, Necropsy analysis post intravenous (I.V.) injection of 15 mg/kg StcE revealed abnormalities in the lung, gastrointestinal tract, and underneath the skull (n = 1 animal). b, SDS-PAGE of plasma and tissues post intraperitoneal (I.P.) injection of PBS or 0.25 mg/kg of IRdye 800CW-labeled StcE (StcE-800, molecular weight = 98 kDa), indicated by the black arrow. c, Anti-mucin Western blot using a catalytically inactive biotin-labeled StcE mutant (biotin-StcEE447D)19 on plasma and tissues from (b). Mucin bands are indicated by black arrows. d, Mucin Western blot on plasma and tissues 3 hours post I.P. injection of PBS or 10 mg/kg StcE. Mucin bands are denoted by black arrows. Replicate gels stained with IRdye 800CW-streptavidin alone (2° only) are shown.
