Fig. 2: Comprehensive neuroanatomical and lymphatic mapping of the whole mouse body using wildDISCO.

a, Depth color coding shows the pan-neuronal marker PGP 9.5⁺ neuronal projections at different z levels in the 2.0 cm-thick whole mouse body. Scale bar, 2,000 μm. b,c, Details of innervation throughout hard (vertebrae) (b) and soft tissues (adipose tissue) (c). Scale bars, 200 μm. d, Optical 2D section showed the PGP 9.5⁺ nerve innervation into multiple organs. Scale bar, 1,200 μm. e, Segmented vagus nerves innervating the kidney (magenta), adrenal gland (green), ureter (cyan), highlighted with specific pseudo-colors. Scale bar, 800 μm. f, Tracing of the TH⁺ vagus nerve over several organs. A single traced vagus nerve masked in magenta from the bottom of the spinal cord to the neck, kidney masked in green and liver masked in cyan. Scale bar, 4,000 μm. g, Higher magnification of the trajectories of the vagus nerve in the mouse can be determined. Scale bar, 1,500 μm. h, A whole mouse stained with a lymphatic vessel marker LYVE1 (yellow). Scale bar, 2,000 μm. i, Lymphoid elements (LYVE1) staining was detected in the brain parenchyma of the mouse. Scale bar, 150 μm. j, Mouse brains stained with two different lymphatic vessel markers (LYVE1 and podoplanin) to identify lymphatic endothelial cells found in the brain regions. Scale bar, 100 μm. b–j, n = 3.