Fig. 3: Different physiological system staining using wildDISCO.
From: Whole-body cellular mapping in mouse using standard IgG antibodies
a, Maximum intensity projection of a mouse stained with antibodies against the TH (green) and the immune cell marker CD45 (magenta), showing the landscape of neuro-immune interactions in internal organs. Scale bar, 1,000 μm. b, The branches of the sympathetic nervous system (TH, green) connect different regions of the intestine. CD45+ cells (magenta) accumulate along parts of the sympathetic nerve, especially at the inferior mesenteric plexus. Scale bar, 200 μm. c, High-magnification views of the labeled regions in a, showing the colocalization of the sympathetic nerve fibers and immune cells on the intestinal wall. Scale bar, 200 μm. d, Representative 2D optical sections of peripheral nerves with immunomodulatory in lymph node (LN) stained with TH and CD45. Scale bar, 100 μm. e, Maximum intensity projections of a whole mouse stained with TH (green) and LYVE1 (yellow). Scale bar, 3,000 μm. f,g, Representative 2D optical sections of hindlimb LNs stained with TH and LYVE1 (f) and PGP 9.5 and Prox1 (g) as indicated in the images to show the LNs are innervated by peripheral nerves with immunomodulatory potential. Scale bars, 150 μm. h, 3D representation of the enteric nerve lattice network of wildtype mice and germ-free mice by immunostaining with antibodies against PGP 9.5. Scale bars, 500 μm. i–l, Higher-magnification views of the regions marked by the white (i), red (j), magenta (k) and yellow (l) boxes. Scale bars, 300 μm. a–l, n = 3. In the germ-free mice, the enteric nerve lattice network appears disorganized, with fewer ganglia. m, The density of the PGP 9.5 enteric plexus was quantified. n = 5; mean ± s.d.; ****P = 3.27 × 10−10, NS, P > 0.05 (one-way analysis of variance).
