Fig. 4: Visualization and analysis of tumor-associated TLS in a tumor metastasis model using wildDISCO and Deep Learning.
From: Whole-body cellular mapping in mouse using standard IgG antibodies
a, 3D rendering of a mouse with 4T1 cell metastases using light-sheet microscopy imaging in ventral view. The TLS are detected and masked in magenta, the tumor cells masked in yellow and the background color is cyan. A higher magnification view shows details of the TLS. Scale bar, 2,000 μm. b–l, Example images of TLS in a mouse with tumors, stained with CD23 in red (b,c) and CD3 in green and CD23 in magenta (d–g). TLS masked in magenta in the primary tumor (h), gut (i,j) and lung (k,l). Scale bars: 500 μm (d,h,k); 150 μm (e,f,g,j); 400 μm (i) and 200 μm (l). m–s, Quantification of the spatial correlation between TLS and metastases throughout the mouse. m, Quantification of the metastasis volume across the mouse. n, Quantification of the metastasis density in lung and gut. n = 4 mice. mean ± s.d. o, Quantification of the distribution of TLS throughout the mouse. n = 4 mice. mean ± s.d. p, Quantification of the TLS volume across the mouse. q,r, Quantification of the distance to nearest neighboring TLS (q), and between metastases and the nearest TLS (r). s, The metastasis volume to the nearest TLS.
