Extended Data Fig. 9: Comparison of editing efficiency of ISAam1 (a) or ISYmu1 (b) relative to five Cas nucleases at three genomic loci in HEK293T cells. | Nature Biotechnology

Extended Data Fig. 9: Comparison of editing efficiency of ISAam1 (a) or ISYmu1 (b) relative to five Cas nucleases at three genomic loci in HEK293T cells.

From: Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors

Extended Data Fig. 9

The gRNA design is shown on the left panel, and editing efficiency shown on the right panel. The seven nucleases are color-coded. Since it is impossible to design overlapping gRNAs targeting the same location across all seven nucleases, two groups of overlapping gRNAs were separately designed for ISAam1, three Cas12f variants and Nme2-C.NR, and for ISAam1 and SaCas9. ISYmu1 was in a similar scenario. Data are shown as the mean ± SD of three biological replicates with actual values overlaid.

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