Extended Data Fig. 2: Information gain with CATS over sparse neuronal labeling.
From: Imaging brain tissue architecture across millimeter to nanometer scales
a, coCATS (gray, xy-STED) labeling by in vivo microinjection into the lateral ventricle of an adult mouse combined with a sparse genetic marker (Thy1-eGFP, yellow, confocal, immunostaining for eGFP) and a synaptic marker (immunostaining for SHANK2, xy-STED) shows the gain in information provided by CATS. b, Magnified view of the yellow box in a, showing mossy fiber boutons, some of which are positively labeled via eGFP expression. When using the sparse genetic marker alone, many synapses (indicated by presence of SHANK2) cannot be assigned within the tissue’s structure. CATS, in contrast, reveals not only the positively labeled, but all cellular structures. Displayed data are from a single dataset representative of coCATS with in vivo microinjection into the lateral ventricle performed in n = 10 animals.
