Extended Data Fig. 2: Targeting modules (T) in organelle-specific pHlicKer variants.
From: Detecting organelle-specific activity of potassium channels with a DNA nanodevice
a, pHlicKerRE localizes in recycling endosomes (REs) by transferrin receptor (TfR)-mediated endocytosis. T is an aptamer that binds TfR. b, pHlicKerTGN is retrogradely trafficked by an scFv-furin chimera to the trans Golgi network (TGN). T is a d(AT)4 sequence (cyan) in pHlicKer that sequence-specifically binds a single chain variable fragment (scFv) fused to the extracellular domain of furin. c, pHlicKerEE localizes in early endosomes (EEs) by scavenger receptor-mediated endocytosis. T is a duplex DNA domain, which is an excellent ligand for scavenger receptors. d–f, Three way (3W) junctions 3WRE, 3WTGN and 3WEE are made from the same sequences as pHlicKerRE, pHlicKerTGN and pHlicKerEE and lack the Alexa488 and TAC-Rh fluorophores for colocalization studies with fluorescent markers of the RE, TGN and EE. g, pHlicKerBiotin incorporates a biotin (grey pentagons) as indicated for immobilization on streptavidin coated beads.
