Extended Data Fig. 3: Calibration of pHlicKer on beads.
From: Detecting organelle-specific activity of potassium channels with a DNA nanodevice
a, Representative images of pHlicKerBiotin on beads clamped at indicated pH and K+ levels, imaged in the donor channel (D), acceptor channel (A), TMR (O), and Alexa Fluor 647 (R) channels. D/A and O/R are the corresponding pixel-wise pseudocolor images. (n = 100 beads). Scale bars, 5 μm. b–d, 2-IM profiles of beads clamped at indicated pH and [K+]. Experiments were performed in triplicate (n = 70–200 beads).
