Fig. 1: Overview of the Quartet Project. | Nature Biotechnology

Fig. 1: Overview of the Quartet Project.

From: Multi-omics data integration using ratio-based quantitative profiling with Quartet reference materials

Fig. 1: Overview of the Quartet Project.

a, Design and production of Quartet family-based multi-omics reference material suites. b, Data generation across multiple platforms, labs, batches and omics types. DDA, data-dependent acquisition; DIA, data-independent acquisition; WGS, whole-genome sequencing. c, QC metrics for horizontal (within-omics) integration include the Mendelian concordance rate and SNR, which are also applicable to wet-lab proficiency testing. Two types of QC metrics for vertical (cross-omics) integration were developed that assess the ability to detect cross-omics feature relationships that follow the central dogma and the ability to classify samples into either four phenotypically different groups (D5–D6–F7–M8) or three genetically driven clusters (daughters–father–mother). d, Ratio-based scaling using common reference materials empowers horizontal and vertical integration.

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