Extended Data Fig. 6: Confirmation of single-cell variants screen using clonal cell-lines. | Nature Biotechnology

Extended Data Fig. 6: Confirmation of single-cell variants screen using clonal cell-lines.

From: Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells

Extended Data Fig. 6

(a) Overview of the confirmation experiments. (b) Violin plot showing the expression of CDKN1A from single-cell sequencing result. P-values are from a two-sided t-test. (c) Identified genetic variants from bulk RNA-seq from isolated clonal cells. (d) Heatmap showing the average GSVA enrichment score of selected pathways on various TP53 mutant HCT116 cell-lines. The V157I mutation was considered as wild-type like from TISCC-seq. (e) Heatmap displays the expression of the 13 genes involved in the p53 pathway. (f) Heatmap shows the expression of 343 genes associated with the P53 pathway.

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