Fig. 2: TISCC-seq identifies mutations directly. | Nature Biotechnology

Fig. 2: TISCC-seq identifies mutations directly.

From: Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells

Fig. 2

a, Overview of single-cell cDNA analysis pipeline. b, Structure of p53 protein and distribution of sgRNA target sites we used in this study. TAD, transactivation domain; PRR, proline-rich region; OD, oligomerization domain; CTD, C-terminal domain. c, Dot plot showing the proportion of each genetic variant detected from single-cell cDNA and genomic DNA. Red dots represent variants with a premature stop codon. d, Cells with same sgRNA can result in various genotypes. The pie chart shows the proportion of resultant amino acid changes from cells with sgRNA targeting V197M mutation. Proportions of mutations are calculated from the single-cell cDNA long-read sequencing. Underlines indicate each triplet codon and numbers indicate position of the codon. Red DNA sequences indicate substituted bases, and blue indicate PAM sequences. WT, wild type.

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