Table 1 Comparison between Argonaute CLIP-seq methods and agoTRIBE
From: Detection of transcriptome-wide microRNA–target interactions in single cells with agoTRIBE
Method | Argonaute CLIP-seq | agoTRIBE |
|---|---|---|
Starting input | Millions of cells | Down to single cells |
Approach | Immunoprecipitation in lysed cells | Editing in living cells |
Antibodies | Needed | Not needed |
Specialized laboratory equipment | Needed | Not needed |
Duration of protocol (excluding cell work) | >4 days | 1.5–2.0 days |
Exact miRNA binding positions | Available | Not available |
Extra information | − | Provides RNA-seq data |
