Extended Data Fig. 1: Morphological evolution of hiPSC-derived aggregates over time.
a, Representative bright field images of day 7 structures derived from WTS2 or WTC11 cell lines and from independent experiments, n = 13 for WTS2 and n = 7 for WTC11. b, Aspect ratio (Length (L)/Width (W)) of WTS2-derived structures over time. At day 7, the mean aspect ratio is 1.7 ± 0.76. c, Distribution of (Lmax-Wmax) of WTS2-derived structures over time: bin center = 50 µm. d, Box-plot of WTC11-derived structures width and length over time. At day 7, the mean length is 516 ± 285 µm. e, WTC11-derived structures aspect ratio over time. At day 7, the mean aspect ratio is 1.7 ± 0.75. f, Distribution of (Lmax-Wmax) of WTC11-derived structures over time: bin center = 50 µm. For box plots, the central line indicates the median; box limits the 1st and 3rd quartiles and whiskers the range. Each dot corresponds to a single structure and different colors indicate independent experiments. For WTS2, n = 11 for day 3; n = 12 for day 4; n = 13 for day 7. For WTC11, n = 2 for day 3; n = 3 for day 4; n = 3 for day 7. Scale bar, 200 µm (a).
