Extended Data Fig. 5: Live imaging of matrigel embedded structures. | Nature Biotechnology

Extended Data Fig. 5: Live imaging of matrigel embedded structures.

From: Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis

Extended Data Fig. 5

a, Time-lapse imaging showing dynamic elongation of matrigel-embedded SOX2-GFP derived structures between day 4 and 5.5. Red arrows pinpoint SOX2 negative cells. b, Time-lapse of matrigel-embedded WTS2 derived structures between day 4 and 7. Post-imaging whole mount immunostaining at day 7 for axial progenitors (SOX2, CDX2) and somitic (FOXC2) markers for the corresponding structure. #3 and #4, examples of non-elongating aggregates. Scale bars, 100 µm (a, b).

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