Extended Data Fig. 2: Survey of screen hits with complementary reporters.
From: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion
a Schematic of luciferase reporters for tethering 100 base pairs away from the splice site. b Clustered bar graph of upstream tethering only hits from the screen comparing results from the original screen (lucMAPT-30U) to results from co-transfection of the RBP-MCP fusions and lucMAPT-100U (mean ± s.d., n = 3 replicate transfections). c Clustered bar graph of downstream tethering only hits from the screen comparing results from the original screen (lucMAPT-30D) to results from co-transfection of the RBP-MCP fusions and lucMAPT-100D (mean ± s.d., n = 3 replicate transfections). d Clustered bar graph of hits that activated both reporters from the screen comparing results from the original screens (lucMAPT-30D, lucMAPT-30U) to results from co-transfection of the RBP-MCP fusions and the long-distance reporters (lucMAPT-100D and lucMAPT-100U) (mean ± s.d., n = 3 replicate transfections). Results where hits displayed a mean ψ from luminescence < 0 are omitted for clarity. e Schematic of lucMBNL1 reporters used as orthogonal exon inclusion reporters. f Bar graphs of reporter readout from co-transfection of all hits from the original screens with lucMBNL1-30D and lucMBNL1-30U (mean ± s.d., n = 3 replicate transfections).
