Extended Data Fig. 3: Exon skipping screen.
From: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion
a Schematic of luciferase reporters for skipping readout. b lucMAP3K7-100U splicing in response to co-transfection with MCP-fused positive and negative controls. (left) Bar graph of lucMAP3K7 reporter readout (mean ± s.d., n = 3 replicate transfections). (right) Agarose gel electrophoresis of RT-generated cDNA amplified by minigene specific primers (shown in panel a) that amplify skipping and inclusion isoforms. c Bar graph of lucMAP3K7-30D reporter readout when co-transfected with RBP-MCP fusions from the library. (mean ± s.d., n = 3 replicate transfections). d Bar graph of lucMAP3K7-100U reporter readout when co-transfected with RBP-MCP fusions from the library (mean ± s.d., n = 3 replicate transfections).
