Fig. 2: Rational design of a monomeric StayGold derivative for use in biological research. | Nature Biotechnology

Fig. 2: Rational design of a monomeric StayGold derivative for use in biological research.

From: A monomeric StayGold fluorescent protein

Fig. 2

a, Size-exclusion chromatography showing the oligomeric state of StayGold and its variants. A mixture of StayGold and mStayGold (that is, E138D) is used to demonstrate their separability. b, Location of two monomerizing mutations (E138D and Y187A) and nonmonomerizing (T195K) mutation in the context of the StayGold dimer. c, Close-up view of the StayGold dimer interface. d, Photostability of StayGold and mStayGold relative to GFP. Fluorescence is given as a percentage of the initial brightness of the S. pombe cytokinetic ring for cells expressing the indicated fluorescent protein fusion to the myosin light chain. Error bars indicate s.d. (n = 20). e, Representative images showing photobleaching for S. pombe cytokinetic ring using GFP, StayGold or mStayGold protein fusions. Yellow triangles indicate the cytokinetic ring.

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